Biofluid Mechanics Research Laboratory


A/Prof. LEO Hwa Liang


Ph.D. (Bioengineering) Georgia Institute of Technology, 2005

M.S. (Mechanical Engineering) Georgia Institute of Technology, 2001

M.Eng. (Mechanical Engineering) Nanyang Technological University, 2000

B.Eng. (Mechanical Engineering) Leeds University, 1996

Appointment Status:

Associate Professor


Department of Biomedical Engineering


Faculty of Engineering,

Block EA, #03-12, 9 Engineering Drive 1,
Singapore 117576.

Office: E2-02-20

Lab: E3-05-18

Phone: +65-6516-5608

Fax: +65-6872-3069


Research Interest:

• Computational and experimental biofluid mechanics

• Heart valve engineering

• Artificial liver device

• In vitro drug screening platform


Selected Publications

Copyright Notice:
The publishers hold the copyright of these articles. The PDFs are provided here to ensure rapid dissemination of scholarly work. It is understood that you will use them only in a manner consistent with the fair-use provisions of the relevant copyright laws. You may not distribute them or use them for any commercial enterprise.

  • Effects of a Novel Carotid Covered Stent on Blood Flow Regime and Hemodynamic Parameters Distribution at the Carotid Artery Bifurcation
    F Kabinejadian, A Danpinid, FS Cui, P Ho, HL Leo. Med Biol Eng Comput, 2015 Feb 53(2):165-77. PDF, Pubmed

  • Comparison of hinge microflow fields of bileaflet mechanical heart valves implanted in different sinus shape and downstream geometry
    YH Kuan, V-T Nguyen, B Su, F Kabinejadian, A Yoganathan, HL Leo. Comput Methods Biomech Biomed Engin, 2014 Oct 24:1-12. PDF, Pubmed

  • In vitro measurements of velocity and wall shear stress in a novel sequential anastomotic graft design model under pulsatile flow conditions
    F Kabinejadian, DN Ghista, B Su, M Kaabi Nezhadian, LP Chua, JH Yeo, HL Leo. Med Eng Phys, 2014 Oct;36(10):1233-45. PDF, Pubmed

  • Comparison of hinge microflow fields of bileaflet mechanical heart valves implanted in different sinus shape and downstream geometry
    YH Kuan, V-T Nguyen, BY Su, F Kabinejadian, A Yoganathan, HL Leo. J Heart Valve Dis, 2014 Sep 23 J Heart Valve Dis

  • Two-dimensional strain-hardening membrane model for large deformation behavior of multiple red blood cells in high shear conditions
    SS Ye, YC ng, J Tan, HL Leo, S Kim. Theor Biol Med Model, 2014 May 13;11:19 Pubmed

  • Effect of erythrocyte aggregation at pathological levels on NO/O2 transport in small arterioles
    S Cho, B Namgung, HS Kim, HL Leo, S Kim. Clin Hemorheol Microcirc, 2014 Apr 14 [Epub ahead of print] Pubmed

  • Design considerations and quantitative assessment for the development of percutaneous mitral valve stent
    GP Kumar, F Cui, HQ Phang, B Su, HL Leo, JK Hon. Med Eng Phys, 2014 Jul;36(7):882-8 Pubmed

  • Has percutaneous aortic valve replacement taken center stage in Aortic Valve Disease treatment
    GP Kumar, F Cui, L Mathew, HL Leo. Crit Rev Biomed Eng, 2013;41(4-5):405-24 Pubmed

  • Perfusion Enhanced Polydimethylsiloxane Based Scaffold Cell Culturing System for Multi-Well Drug Screening Platform
    M Tania, MN Hsu, SN Png, HL Leo, GW Toh, E Birgersson. Biotechnol Prog, 2014 Mar-Apr;30(2):418-28 Pubmed

  • Computational Fluid Model Incorporating Liver Metabolic Activities in Perfusion Bioreactor
    MN Hsu, G-D S Tan, M Tania, E Birgersson, HL Leo. Biotechnol Bioeng, 2014 May 111(5):885-895 Wiley

  • Numerical investigation of blood flow in three-dimensional porcine left anterior descending arterial tress with various stenoses
    B Su, Y Huo, GS Kassab, F Kabinejadian, S Kim, HL Leo, L Zhong. Comput Biol Med, 2014 Apr;47:130-8 Pubmed

  • Numerical Simulation of Patient-specific Left Ventricular Flow with both Mitral and Aortic Valves by FSI Approach
    B Su, L Zhong, XK Wang, JM Zhang, RS Tan, JC Allen, SK Tan, S Kim, HL Leo. Comput Methods Programs Biomed, 2014 Feb;113(2):474-82 Pubmed

  • Enhanced and conventional project-based learning in an engineering design module
    KJ Chua, WM Yang, HL Leo. Int J Tech Design Education, 2014 Nov;24(4):437-458 Springer

  • Scalable alignment of three-dimensional cellular constructs in a microfluidic chip
    CG Anene-Nzelu, KY Peh, A Fraiszudeen, YH Kuan, SH Ng, YC Toh, HL Leo, H Yu. Lab Chip, 2013 Oct 21;13(20):4124-33. PDF, Pubmed

  • A Novel Carotid Covered Stent Design: In Vitro Evaluation of Mechanical Performance and Influence on the Blood Flow Regime at the Carotid Artery Bifurcation
    F Kabinejadian, A Danpinid, FS Cui, P Ho, HL Leo. Ann Biomed Eng, 2013 Sep;41(9):1990-2002. Pubmed

  • Current hydrogel solutions for repairing and regeneration of complex tissue
    Y Wang, LQ Cai, B Nugraha, Y Gao, HL Leo. Curr Med Chem, 2014;21(22):2480-96. Pubmed

  • Effect of deformability difference between two erythrocytes on their aggregation
    M Ju, SS Ye, HT Low, J Zhang, P Cabrales, HL Leo, S Kim. Phys Biol, 2013 Jun;10(3):036001. Pubmed

  • Design and Finite Element Based Fatigue Prediction of a Novel Percutaneous Mitral Valve Stent’, Computer-Aided Design
    GP Kumar, F Cui, A Danpinid, B Su, FH Kim, HL Leo. Computer-Aided Design, 2013 Oct;45(10):1153-1158. ACM Digital Library

  • A semi-automated method for patient-specific computational flow modelling of the left ventricle
    V-T Nguyen, JL Chong, HH Nguyen, Z Liang, HL Leo. Comput Methods Biomech Biomed Engin, 2015;18(4):401-13. PDF, Pubmed

  • Scalable cell alignment on optical media substrates.
    CG Anene-Nzelu, D Choudhury, H Li, A Fraiszudeen, KY Peh, YC Toh, SH Ng, HL Leo, H Yu. Biomaterials, 2013 Jul:34(21):5078-87. PDF, Pubmed

  • A thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture.
    GD Tan, GW Toh, E Birgersson, J Robens, D van Noort, HL Leo, Biotechnol Bioeng. 2013 Jun;110(6):1663-73 PDF, Pubmed

  • Computational fluid modeling and performance analysis of a bidirectional rotating perfusion culture system.
    Kang CW, Wang Y, Tania M, Zhou H, Gao Y, Ba T, Tan GD, Kim S, Leo HL. Biotechnol Prog. 2013 Apr 1 PDF, Pubmed

  • Experimentally Validated Hemodynamics Simulations of Mechanical Heart Valves in Three Dimensions.
    V-T Nguyen, YH Kuan, P-Y Chen, L Ge, F Sotiropoulos, AP Yoganathan, HL Leo. Cardiovascular Engineering and Technology, 2012 Mar:3(1):88-100. PDF, Springer

  • Recent Advances in Transcatheter Heart Valve Replacement: A Review on Aortic and Mitral Implantation.
    M Ismail, JKF Hon, ZW Chan, HL Leo. Recent Patents in Biomedical Engineering, 2012 June; 5: 235-52. PDF, Bentham Science

  • Recent Advances in Polymeric Heart Valves Research (Review).
    YH Kuan, LP Dasi, AP Yoganathan, HL Leo. International Journal of Biomaterials Research and Engineering, 2011 Volume 1, Issue 1 PDF, IGI Global

  • A robust high-throughput sandwich cell-based drug screening platform.
    Zhang S, Tong W, Zheng B, Susanto TA, Xia L, Zhang C, Ananthanarayanan A, Tuo X, Sakban RB, Jia R, Iliescu C, Chai KH, McMillian M, Shen S, Leo HL, Yu H. Biomaterials. 2011 Feb;32(4):1229-41. doi: 10.1016/j.biomaterials.2010.09.064. Epub 2010 Oct 23 PDF, Pubmed

  • Current development of bioreactors for extracorporeal bioartificial liver (Review).
    Wang Y, Susando T, Lei X, Anene-Nzelu C, Zhou H, HL Leo, Yu H. Biointerphases. 2010 Sep;5(3):FA116-31. doi: 10.1116/1.3521520. PDF, Pubmed

  • Microfabricated silicon nitride membranes for hepatocyte sandwich culture.
    Zhang S, Xia L, Kang CH, Xiao G, Ong SM, Toh YC, Leo HL, van Noort D, Kan SH, Tang HH, Yu H. Biomaterials. 2008 Oct;29(29):3993-4002. doi: 10.1016/j.biomaterials.2008.06.024. Epub 2008 Jul 17. PDF, Pubmed

  • Spatio-temporal flow analysis in bileaflet heart valve hinge regions: potential analysis for blood element damage.
    Simon HA, Dasi LP, Leo HL, Yoganathan AP. Ann Biomed Eng. 2007 Aug;35(8):1333-46. Epub 2007 Apr 13. PDF, Pubmed

  • Effect of hinge gap width on the microflow structures in 27-mm bileaflet mechanical heart valves.
    Leo HL, Simon HA, Dasi LP, Yoganathan AP. J Heart Valve Dis. 2006 Nov;15(6):800-8. PDF, Pubmed

  • The angle-resolved velocity measurements in the impeller passages of a model biocentrifugal pump.
    SCM Yu, LP Chua, HL Leo. Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science May 1, 2001 vol. 215 no. 5 547-568.
    PDF, SAGE Journal